DL-Serine Assay Kit

DL-Serine Assay Kit

The non-proteogenic isomer D-Serine may also be synthesized from L-Serine inside the mammalian thoughts. Whereas most D-amino acids are solely present in trace portions in mammals, D-Serine is a crucial neurotransmitter that acts as a co-agonist of synaptic N-methyl-D-aspartate (NMDA) kind glutamate-gated ion channels. Impaired D-Serine metabolism would possibly contribute to a lot of neuropsychiatric issues, along with melancholy, schizophrenia, epilepsy and dementia. BioVision’s DL-Serine Assay Package deal permits for quantification of every L- and D-Serine in natural fluids and tissues.

DESCRIPTION

The assay relies on the conversion of L-Serine to D-Serine, which is metabolized to an intermediate product that is subsequently oxidized and reacts with a probe to form a gradual fluorophore (Ex/Em = 539/587 nm). Samples may be divided and assayed concurrently for quantification of every D-Serine and full DL-Serine. The assay is simply not affected by physiological concentrations of various amino acids, is high-throughput adaptable and should detect decrease than 1 μM Serine

Serine is among the many 20 naturally-occurring amino acids utilized by all organisms inside the biosynthesis of proteins. Having a single chiral center, serine can exist as each of two stereoisomers (L-Serine and D-Serine).

DL Serine Standard Curve
DL Serine Standard Curve
Cat # +Measurement Okay743-100
Measurement 100 assays
Package deal Summary • Detection method- Fluorescence (Ex/Em 535/587 nm)
• Software program- Measurement of Serine in natural samples harking back to serum, plasma, CSF, and so forth.
Detection Methodology Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Mammalian
Functions Estimation of Serine in diversified natural samples
Choices & Benefits • Simple, quick & helpful assay
• Prohibit of detection: 1 µM
• Very specific, does not work along with totally different regular and non-standard amino acids
Package deal Components • Serine Assay Buffer
• Probe Reply
• Serine Racemase Enzyme Mix
• D-Serine Enzyme Mix
• Developer Enzyme Mix
• Sample Cleanup Mix
• D-Serine Commonplace
Storage Conditions -20°C
Supply Conditions Gel Pack
USAGE For Evaluation Use Solely! Not For Use in Individuals.

 

 

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